B95 8 Cell Line

However the cell line is derived from a primate species that is listed as endangered by the u s.

B95 8 cell line.

Through the use of dna profiling it has now been shown. This cell line is used as a source of ebv. B95 8 cell line flask presentation1 flask 75 cm2 ftb95 information request. B95 8 b95 8 b958 cotton top tamarin monkey peripheral blood lymphocyte derived from a cotton top tamarin saguinus oedipus.

This has been confirmed by dna profiling. The viral strain was isolatedfrom a case of transfusion induced mononucleosis and subsequently used in vitro to immortalise monkey blood leukocytes miller et al pnas 1972 to generate the cell line. Many investigators look for b95 8 cells atcc crl 1612 because this monkey cell line sheds the epstein barr virus that is used in transforming b lymphocytes. The cells should be handled under laboratory containment level 2.

B95 8 marmoset leukocyte cell line supplied by atcc used in various techniques. B95 8 monkey marmoset lymphocyte b peripheral species. 94 100 based on 2 pubmed citations. Thus it provides a source of ebv to establish continuous lymphocytic lines from human donors.

This cell line is used as a source of ebv. Releases high titres of transforming ebv. In some instances the cell line b95 8 has been described as. Marmoset used to be described in the animal name section was a mistake and corrected to the name cotton top tamarin with the scientific name.

Fish and wildlife service. In some instances the cell line b95 8 has been described as derived from marmoset however this is not correct. If you need more information about the product please fill in the form. The ebv producing marmoset b cell line b95 8 commonly used as a source of ebv for stimulation and transformation of human b cells was shown to proliferate in response to supernatants containing human b cell growth factors bcgf derived from pha activated t cells or the kg la cell line and to a commercial low molecular weight bcgf bcgflow but not to recombinant human il 4.

The b95 8 cell line must be handled in the bsl 2 labotory since ebv can infect primates. Zero bias scores article reviews protocol conditions and more. The b95 8 cell line s name originates from the b95 strain of epstein barr virus ebv. This cell line has been assigned by the zkbs german central commission for biological safety to risk group 2 ief analysis with ldh and np confirmed the cells as non.

The b95 8 cell line must be handled in the p2 labotory since ebv can infect primates. B95 8 was derived from a cotton top tamarin saguinas oedipus.